Development of a highly sensitive three-dimensional gel electrophoresis method for characterization of monoclonal protein heterogeneity
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چکیده
منابع مشابه
Development of a highly sensitive three-dimensional gel electrophoresis method for characterization of monoclonal protein heterogeneity.
Three-dimensional gel electrophoresis (3-DE), which combines agarose gel electrophoresis and isoelectric focusing/SDS-PAGE, was developed to characterize monoclonal proteins (M-proteins). However, the original 3-DE method has not been optimized and its specificity has not been demonstrated. The main goal of this study was to optimize the 3-DE procedure and then compare it with 2-DE. We develope...
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ژورنال
عنوان ژورنال: Analytical Biochemistry
سال: 2013
ISSN: 0003-2697
DOI: 10.1016/j.ab.2013.03.013